Figure 4
Effects of A 1 R activation on preBötC rhythmogenesis in island preparations. A. Representative effects of NCPA (1 μM) alone, with gabazine (20 μM) and in combination with 20 μM gabazine and 1 μM strychnine. B. As with slice preparations, NCPA alone (i) or in combination with gabazine (ii) decreased burst frequency. By contrast to slices, NCPA applied in combination with gabazine and strychnine (iii) failed to affect burst frequency (a, value different from baseline at P < 0.05, Tukey post-hoc test; b, value different from the second step of treatment – either continued aCSF, application of gabazine, or application of gabazine and strychnine – at P < 0.05, Tukey post-hoc test).