Figure 5

Real time quantitative RT-PCR from human donor ONH astrocytes. qRT-PCR was performed using mRNA isolated from primary cultures of ONH astrocytes derived from nine donors with primary open angle glaucoma (CAG) and nine age-matched Caucasian American normal donors (CA; Supplemental Table 2). Clear bars indicate control samples (CA) and filled-in bars indicate glaucomatous samples (CAG). Values represent the mean ± SE of relative expression normalized to 18S of each group of normal and glaucomatous astrocytes. For each donor, reactions were carried out in triplicate. A. GPNMB, fold change = 16.81; p = 0.0004. B. RBP1, fold change = 6.02; p = 0.02. C. CAPG fold change = 2.55; p = 0.02. D. TIMP1, fold change = 1.77; p = 0.02. E. APOE, fold change = 17.91; p = 0.04. F. TGFBI, fold change = 1.58; p > 0.05.