Labeling with antiserum to sNPFp and OK371- or TH -Gal4-driven GFP in the larval CNS. These Gal4 lines display expression of vesicular glutamate transporter (vGluT) and tyrosin hydroxylase, respectively. A1 – A2. Some colocalization of vGluT and sNPF was seen in a cell body group in the dorso-lateral protocerebrum (DLN; see Fig. 1E). A magnified view is shown in inset (arrows indicate cell bodies with colocalized markers). The beta-lobe (βL) of mushroom body is surrounded by OK371-expressing processes. B. More dorsally in the brain another cell body co-expresses markers (arrow). Ca, calyx of mushroom body. C – E. In the ventral nerve cord (abdominal ganglia shown here; anterior downwards) vGluT and sNPF appear not to be colocalized. In C1 and C2 sNPF-IR processes are seen in a dense terminal plexus neuropil of A8/9 (arrow), that is not supplied by vGluT expressing fibers. In D and E it can be seen that sNPF-IR cell bodies are located in clusters with vGluT expressing ones (arrows). In the more dorsal focal plane in E it is apparent that varicose sNPF-IR fibers run through neuropils with dense supply of vGluT expressing processes. F. Tyrosin hydroxylase expression (TH-Gal4) is seen in a pair of cell bodies in each of the abdominal ganglia A1 – A7 (green). These cell bodies are clustered with sNPF-IR cell bodies (e. g. at arrow), but no colocalization is seen anywhere in the CNS.