Figure 11

Larval sNPFp immunoreactive neurons do not coexpress DIMM. Double labeling of larval CNS with antiserum to sNPFp (magenta) and dimm -Gal4-driven GFP (c929-Gal4), voltex projections. A. Brain, subesophageal ganglion (SEG) and thoracic ganglia (ThG) with dimm-expressing neurons distinct from sNPFp-expressing ones. In the DLN cluster (arrows) in the brain corazonin-immunoreactive neurons (see Fig. 6C) can be found. These sNPF-corazonin expressing neurons could possibly coexpress dimm [15] (but GFP may be too weak here to be seen). MNC, median neurosecretory cells; MB, mushroom body lobes; Ca, calyx. B. Higher magnification of right brain hemisphere of another larva with the sNPFp-expressing DLN cluster of cell bodies and dimm-expressing median neurosecretory cells (MNC). No dimm-expression is seen in any of these neurons. C – D. Double labeling with anti-sNPFp and c929-Gal4 in larval ventral nerve cord shown in maximum projections and voltex reconstruction. No colocalization of markers is seen. C. Ventral view of the segments A3 – A9. D. Dorsal view of the segments A1 – A2. E. Cross section (rear view) of the segments A1 – A2.