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Figure 3 | BMC Neuroscience

Figure 3

From: Enhanced expressions of microvascular smooth muscle receptors after focal cerebral ischemia occur via the MAPK MEK/ERK pathway

Figure 3

Immunofluorescence staining for pERK1/2, and transcription factor pELK-1 in the ischemic middle cerebral artery (MCA), cerebral microvessels (Mic.V), and surrounding brain tissue (Brain) in rats treated with vehicle, MCAO + vehicle, or MCAO plus U0126 at different time points. There was an increase in activated (phosphorylation = p) ERK1/2 and ELK-1 in the smooth muscle cell layer of ischemic vessels (MCA and Mic.V) as compared to vehicle. Treatment with U0126 starting at 0 h, 6 h and 12 h after occlusion prevented the increase in expression of pERK1/2 in smooth muscle cells of the MCA and Mic.V as studied at 48 h. Data were obtained with confocal microscopy. Scale bar, 50 μm.

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