Western blot analysis of caspase-3 expression in Hcy-treated and untreated Neuro2a cells. After a 4 h Hcy treatment, in the presence or absence of the specific NF-κB inhibitor SN50, cell proteins were probed with monoclonal antibodies against caspase-3 and β-actin (figure top). A densitometric analysis of immunoblots (figure bottom) was also carried out after normalization against β-actin. Results were similar in three separate experiments. *p < 0.05 significant difference in comparison with untreated cells; §p < 0.05 in comparison to Hcy-treated cells.