Effects of antidepressant treatment on expression of total BDNF mRNA and BDNF transcripts in SH-SY5Y cells. A. Expression of total BDNF mRNA in SH-SY5Y cells after 6 h, 24 h and 48 h treatment with 10 μM fluoxetine (FLX), reboxetine (RBX) or desipramine (DMI), relative to untreated cells. Data are expressed as % intensity units/mm2 vs. untreated cells (mean ± s.e.m.). Statistics: two-way ANOVA, Bonferroni post-hoc test vs. untreated cells: *p < 0,05 ***p < 0,001 RBX vs. untreated cells; §p < 0,05 §§§p < 0,001 DMI vs. untreated cells. B. Expression level of BDNF IV transcript after 6 h and 48 h treatment with 10 μM FLX, RBX or DMI, relative to untreated cells. *p < 0.05 ***p < 0.001 RBX vs. untreated cells; §§p < 0.01 DMI vs. untreated cells. C. Expression level of BDNF VIa/b after treatment as in (B). Data and statistics as above. ***p < 0.001 RBX vs. untreated cells.