|
Cerebral
|
Neuroblastoma
|
RGC-5
|
---|
|
N = 7
|
N = 7
|
N = 8
|
---|
|
Mean
|
SEM
|
Mean
|
SEM
|
Mean
|
SEM
|
---|
Mitochondria
|
0.193
|
0.027
|
0.348
|
0.042
|
0.028
|
0.005
|
Succinate
|
0.216
|
0.022
|
0.307
|
0.024
|
0.031
|
0.005
|
Antimycin A
|
0.540
|
0.116
|
0.277
|
0.034
|
0.024
|
0.004
|
- The rates of superoxide production were determined after the addition of the complex II substrate succinate (10 mM) and the complex III inhibitor antimycin A (0.5 μM). Superoxide was indirectly measured using the H2O2 probe Amplex Red in the presence of horseradish peroxidase (HRP). The values are given in nmol H2O2/min/mg protein ± standard error of the mean (SEM), with n being the number of independent samples for each mitochondria type.