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Table 5 pTHlac or pINS-TH-NFHlac support expression for 1 month after targeted gene transfer to nigrostriatal neurons using either gC – bdnf or gC – gdnf, respectively

From: Enhanced nigrostriatal neuron-specific, long-term expression by using neural-specific promoters in combination with targeted gene transfer by modified helper virus-free HSV-1 vector particles

  

Numbers of positive cells

% Long-term expression*

  

4 days

1 month

  

Vectors

gC

β-gal-IR

Flag-IR

β-gal-IR

Flag-IR

β-gal

Flag

pTHlac & pHSVpkcΔGG

gC – bdnf

2,071 ± 602

1,723 ± 284

768 ± 36

478 ± 38

37

28

pINS-TH-NFHlac & pHSVpkcΔGG

gC – gdnf

2,230 ± 402

2,214 ± 209

816 ± 121

515 ± 94

37

23

  1. Using pTHlac & pHSVpkcΔGG, 4 rats were analyzed at 4 days, and 2 rats were analyzed at 1 month. Using pINS-TH-NFHlac & pHSVpkcΔGG, 3 rats were analyzed each time point. The means ± sems are shown.
  2. *% long-term expression = β-gal-IR cells at 1 month/β-gal-IR cells at 4 days × 100.
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BMC Neuroscience

ISSN: 1471-2202

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