Enhanced nigrostriatal neuron-specific, long-term expression by using neural-specific promoters in combination with targeted gene transfer by modified helper virus-free HSV-1 vector particles

Table 4 The numbers of β-gal or PkcΔGG positive cells that contained TH-IR, in rats sacrificed at 1 month after co-injection of pINS-TH-NFHlac/gC – gdnf and pHSVpkcΔGG/gC – gdnf into the midbrain

	Numbers of positive cells		%	Numbers of positive cells		%
Rat	β-gal-IR	TH-IR	Costained	Flag-IR	TH-IR	Costained
1	240	223	93	156	122	78
2	144	132	92	148	88	60
3	228	210	92	82	56	68
Average			92 ± 0			69 ± 5

This experiment was performed as in Table 1, except a mixture of equal titers of pINS-TH-NFHlac/gC – gdnf and pHSVpkcΔGG/gC – gdnf was injected into the midbrain, and the rats were perfused at 1 month after gene transfer.

ISSN: 1471-2202