Table 1 Composition of culture media
Supplements in DMEM | |||||||
|---|---|---|---|---|---|---|---|
Day of culture | Culture condition | Description of media | FCS (15%) | RA (10 μM) | BDNF (5 ng/ml) | HSA (0.1%) | Ham's F12 |
Seeding | Normoxia | Maintenance medium (MM) | x | - | - | - | - |
1 – 4 | Normoxia | Basic medium (BM) | x | x | - | - | - |
5 – 16 | Normoxia | Differentiation medium (DM) | - | x | x | x | x |
16 – 18 | Normoxia/hypoxia | Differentiation medium (DM) | - | x | x | x | x |
18 – 21 | Normoxia | Post-hypoxic medium (PHM) | - | - | x | x | x |