Figure 1
From: The type III neurofilament peripherin is expressed in the tuberomammillary neurons of the mouse
In situ hybridization of peripherin. The expression of peripherin mRNA was studied with ISH. The autoradiogram in A shows a mouse brain section hybridized with a 35S-labelled peripherin probe. The selective staining of the TM nucleus is evident. In B, a digoxigenin labelled mRNA probe reveals the distribution of peripherin expressing neurons in detail. The distribution is very similar to the histaminergic TM neurons. In C, neurons of the lateral TM that were hybridized simultaneously with a digoxigenin labelled probe for peripherin and a 35S-labelled probe for HDC are shown. The alkaline phosphatase staining for peripherin, which differentiate the neurons from the background, is colocalized with the silver grains that indicate the localization of HDC expression. 3 V, third ventricle. Scale bar 100 μm in B, 20 μm in C.