Figure 4

Neurosphere cells could differentiate into SC-like cells. Neurosphere cells were cultured for 48 hours on poly-L-lysine-coated six-well chamber slides in SC differentiation medium. A, B: Under phase contrast, most of the differentiated neurosphere cells (SC-like cells) are bi- or tri-polar, spindle-like 48 hours after differentiation (A), and these spindle-like cells can proliferate to a higher cell density when the SC differentiation medium was replaced with DMEM plus 10% FBS 48 hours after differentiation (B). C-F: Immunocytochemistry of SC-like cells. Almost all the SC-like cells express nestin (C), GFAP (D) and S-100 (E) in the cytoplasm, and 35 ± 5% of the SC-like cells express p75 in the cytomembrane (F). Nuclei are labeled with DAPI (blue). Bar, 50 μm.