Gαq-family immunoreactivity co-localizes with T1R3-GFP. In vallate papilla TBs, the Gq/11/14 antibody (red) stains most of T1R3-GFP (green) cells (30 of 41). Fewer cells are strongly positive for Gαgus (blue). The large majority of Gq/11/14-IR cells (30 of 35) exhibit T1R3-GFP. Only about half of GαGus IR cells show T1R3-GFP expression). The figure in Additional file 3 shows single plane confocal images from this image set for comparison. Scale bar 20 μM.