Insulin sensitivity is maintained during anaesthesia if hypothermia is prevented. Mice were injected i.p. with ketamine/Xylazine (K/X), and body temperature maintained at 37°C. Half of the group (n = 4) were given insulin by icv injection, the other half (n = 4) received saline 30 mins prior to sacrifice and rapid removal and dissection of the brain. Equal amounts of cortical protein were subjected to western blot using the antibodies shown (A), and results from each treatment group quantified by densitometry (B). The mean ± SD, relative to the control animals, is shown. Significant differences between control and each treatment group are given, *p < 0.05, **p < 0.005. Phosphorylation of ERK and PKB was also compared between animals sacrificed without anaesthesia and those with anaesthesia but whose body temperature was maintained at 37°C (C).