Figure 2

Effect of IL-3 on fibrillary Aβ-induced neurotoxicity in cortical neurons. MTT, Tripan blue and TUNEL staining analyses were used to determine cell death. (A). Cortical neurons were treated with different concentrations of Aβ peptide for 24 h at 37°C. (B). Neurons were incubated with different concentrations of IL-3 and then treated with 10 μM Aβ. (C). Neurons were incubated with different concentrations of IL-3 in the absence of Aβ. (D). Neurons were incubated with different concentrations of IL-1 and then treated with 10 μM Aβ. Data are mean ± S.E. for three separate experiments performed in duplicate. (E). Cortical neurons were treated with 10 μM Aβ in the absence or presence of 5 nM IL-3 for 24 h at 37°C. TUNEL-positive neurons were visualized by microscopy and the shrunken nuclei are indicating by arrows. (F). Primary cortical neurons were pre-treated with 5 nM IL-3 or 100 μM insulin for 30 min, and then treated with 10 μAβ for 24 h at 37°C. MTT and Tripan blue analyses were used to determine cell death. Data are means ± S.E. for three separate experiments performed in duplicate.