Figure 5

Immunohistochemical analysis of PSEN1 and PSEN2 expression in embryonic E100 porcine brains. Coronal brain sections were immunohistochemical stained for PSEN1 or PSEN2 and nuclei counterstained by haematoxylin. Sections illustrating PSEN2 (left sections) and PSEN1 (right sections) staining patterns in hippocampus (A), cerebral cortex (B), and cerebellar cortex (C) are shown for embryonic day E100. The upper sections in panel A, B and C were magnified ×20 and the lower sections ×160. The regions enlarged in the lower sections are approximately indicated by the squares in the upper sections. The enlarged region in panel A corresponds to hippocampal pyramidal neurons (PN) of the CA3. The hippocampal dentate gyrus (DG) is indicated in the upper sections. The enlarged region in panel B corresponds to cortical neurons (CN) of the cerebral cortex. The enlarged region in panel C shows the molecular layer (ML), Purkinje neurons (PN), and the granular cell layer (GCL) of the cerebellar cortex.