Figure 8
Summary of the quantitative analysis of calcium signal spreading within a compartment and across the nuclear envelope. Exponential curve fits were used to calculate the time constants at half maximum, tFmax/2, for the calcium imaging traces measured at the various distances from the uncaging spot. A and B, an example of a mathematical fit and the corresponding experimental data is shown; in the example, the uncaging spot was localized to the nucleus and calcium was measured in the cytosol at a distance of 5 μm from the uncaging spot. Scale bar is 10 μm. C through F, summary of all tFmax/2 values for calcium imaging traces measured within a compartment and across the nuclear envelope at the various distances from the uncaging spot; calcium imaging data were acquired at 2.5 Hz (C and D) and 11.6 Hz (E and F). Bars represent means ± s.e.m. (3 independent experiments; at each distance, 5 to 8 cells were analyzed in every experiment).