Figure 1
From: Sodium channel Nav1.6 accumulates at the site of infraorbital nerve injury
Na v 1.6 and caspr staining. A and B. Confocal micrographs from average-intensity z-projections (45 slices in A; 47 slices in B) demonstrate Nav1.6 (red) and caspr (green) staining relationships seen within the normal (A) and lesioned (B) ION. A. The normal ION shows bright Nav1.6 staining (arrowhead) at nodes of Ranvier that are identified as the gap seen between the paranodal staining of caspr. The caspr staining is intense within the paranodal region and prominent Nav1.6 staining is generally restricted to the narrow area at the node. B. The lesioned ION, at a location just proximal to the chromic suture, shows a change in the Nav1.6 and caspr staining relationships that were seen in A. These changes include a loss in the overall organization and the immunofluorescence intensity of caspr staining within the paranodal region (arrowheads) and an increased size and intensity of Nav1.6 within accumulations (large arrows). Alterations in the normal Nav1.6 and caspr staining relationships include Nav1.6 accumulations that show an association with caspr on one side (heminodes; small arrow). Both images were collected with the same laser settings. Scale bars = 10 μm.