Confocal laser scanning microscopy (CLSM) images of α-gustducin-LIR (α-gustducin) in taste buds of rat and mouse circumvallate papillae. Longitudinal sections show a subset of taste cells displaying α-gustducin-LIR in rat (A) and mouse taste buds (B). The α-gustducin-LIR taste cells are spindle-shaped with large, round nuclei. Transverse sections show α-gustducin-LIR in rat (C-E) and mouse taste buds (F-H). The immunoreactivity is only cytoplasmic in both transverse and longitudinal sections. Immunoreactive taste cells (red) are shown in C and F. Sytox-stained nuclei (green) are shown in D and G and merges are shown in E and H. Scale bars = 20 μm.