Figure 5

Expression of HIV-1 regulatory and structural genes by infected HFA and control cells. A) HFA were infected with VSV-G pseudotyped NL4-3 encoding a NefGFP fusion protein (first row), with native NL4-3 encoding NefGFP (second row) or encoding RevGFP (third row). Two days after infection HFA were stained using specific antibodies for GFAP (first and second rows) or p24 (third row) and with DAPI and Texas Red-labeled secondary antibodies. Images of red and green fluorescence were superimposed (third column). B) MAGI cells were infected with NL4-3 encoding NefGFP (first row), or fusion incompetent 517B encoding NefGFP (second row), or 517B-NefGFP pseudotyped by VSV-G (third row). Two days after infection cells were stained with anti-actin, DAPI, and Texas Red-labeled secondary antibody and images of red and green fluorescence were superimposed (third column).