Figure 6From: Requirement of aggregation propensity of Alzheimer amyloid peptides for neuronal cell surface bindingFlow cytometry fluorescence results indicating trypsin significantly reduces Aβ cell surface binding. (A) Cell surface treatments show that 10 μg/mL trypsin significantly decreased Aβ42 association to NGF differentiated PC12 cells, while treatments that target cell surface lipids or carbohydrates had no effect. SBTI-inactivated trypsin had little effect on Aβ42 association. (B) Concentration dependence of trypsin treated NGF differentiated PC12 cells with 0, 5, 10 and 100 μg/mL trypsin as indicated, followed by 1 hour Aβ42 treatment. (C) 10 μg/mL trypsin treatment of NGF differentiated PC12 cells demonstrate that Aβ42 association with the cell surface recovers over time.Back to article page