Figure 2

In situ hybridization analysis of muskelin transcripts in newborn (P0) and adult stage (P42). (A) Postnatal stage P0. Left: basal transcript expression is found throughout the brain with signals in the hippocampal formation and the cerebellum above average (arrows). Middle: at the newborn stage transcripts are also prominent in the spinal cord (arrows). Right: The sense control displays a parallel experiment using sense RNAs as probe. (B) Adult stage. Basal muskelin transcript expression is found throughout the brain. Hybridization signals in cerebellum and hippocampus are particularly strong, as compared to other brain regions. The sense control displays a parallel experiment using sense RNAs as probe. In the hippocampus, muskelin transcripts display highest concentration in cell bodies of pyramidal cells in CA1 and CA3 regions, as well as in cell bodies of dentate gyrus granule cells. In the cerebellum, muskelin transcripts are mainly found in cell bodies of granule and Purkinje cells. Key: CA1, Hippocampal CA1 region; CA3, Hippocampal CA3 region; Cb, Cerebellum; Cx, Cortex; DG, Dentate gyrus; GCL, Granule cell layer; Hc, Hippocampus; ML, Molecular layer; Ob, Olfactory Bulb; WM, White matter. Scale bars: (A) and (B) 1 mm.