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Table 3 Quantitative real-time PCR (qPCR) does not support Sema3A-induced changes in DRG observed by microarray.

From: Analysis of gene expression during neurite outgrowth and regeneration

Gene Gene Title Max. difference by microarray§ Max. difference by qPCR p-value
Cntn3 Contactin 3 2.1 2.6 0.34
Col18 Procollagen, type XVIII, alpha 1 1.6 2.5 0.38
Fdps Farnesyl diphosphate synthetase 1.7 1.4 0.18
Gli2* GLI-Kruppel family member GLI2 1.5 1.8 0.66
Tubb3 Tubulin, beta 3 1.3† 1.7 0.01
  1. p-value indicates significance of qPCR data for a quadratic model of change over time. Italicized p-values indicate lack of statistical significance.
  2. * Selection of these genes included probesets not part of the matched DRG-SCG dataset, as an additional screen for genes missed by these filters.
  3. § Mock-Sema3A differences observed by microarray were statistically significant (quadratic model; p ≤ 0.05).
  4. † By microarray analysis, the mock-Sema3A differences observed for Tubb3 were not statistically significant.
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BMC Neuroscience

ISSN: 1471-2202

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