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Table 1 Validation using quantitative real-time PCR (qPCR) of genes found to change significantly in DRG by microarray.

From: Analysis of gene expression during neurite outgrowth and regeneration

Gene Gene Title Max. fold change by microarray§ Max. fold change by qPCR p-value
Cntn3 Contactin 3 2.6 11.0 0.007
Col18 Procollagen, type XVIII, alpha 1 4.3 8.1 0.0003
Cryab* Crystallin, alpha B 123.0 42.5 <.0001
Fdps Farnesyl diphosphate synthetase 2.4 2.0 <.0001
Gli2* GLI-Kruppel family member GLI2 1.8 2.4 0.03**
Sparc Secreted acidic cysteine rich glycoprotein 4.8 6.5 <.0001
Timp3* Tissue inhibitor of metalloproteinase 3 2.6 3.6 0.003
Tubb3 Tubulin, beta 3 1.6 2.6 0.0002
  1. p-value indicates significance of qPCR data for a quadratic model of change over time.
  2. * Selection of these genes included probesets not part of the matched DRG-SCG dataset, as an additional screen for genes missed by these filters.
  3. ** Significance for this gene is for a linear model of change over time; this gene does not fit a quadratic model.
  4. § Changes observed by microarray were statistically significant (quadratic model; p ≤ 0.05).