Figure 9

GAP-43 mRNA expression after LPS application. Coronal sections of motor cortex hybridised with GAP-43 mRNA probe 3 days (Figs 9a – d), 7 days (Figs 9e, f) and 1 month (Figs 9g, h) after unilateral application of LPS (Figs 9b, d, f, h) or sham operations to the contralateral (control) side (Figs 9a, c, e, g). Background levels of GAP-43 mRNA are seen in contralateral (control) cortex at 3 days but stronger expression is apparent in layers II–V of LPS-treated cortex. Areas of layer V in Fig. 9a and b are enlarged in Fig. 9c and d to better show differences in hybridisation signals. By 7 days (Figs 9e and 9f), GAP-43 mRNA expression appears to be identical on both sides, and remains so one month after LPS application (Figs 9g and h). Scale bar in Fig. 9a = 500 μm and also applies to Fig. 9b; scale bar in Fig. 9c = 50 μm and also applies to Fig. 9d; scale bar in Fig. 9e = 200 μm and also applies to Figs 9f – h.