Figure 1

17β-estradiol prevents Amyloid beta_1–42-induced neurotoxicity. Primary hippocampal neurons pretreated with E₂ (10 ng/mL; 48 hr) or vehicle control were exposed to Aβ_1–42 for 3 days and assessed for neuronal survival by calcein/ethidium homodimer staining. A, Visualization of viable (green) and dead (red) neurons. Bar = 20 μm. B-C, Fluorometer readings of calcein (live) and ethidium homodimer (dead) signals. (** = p < 0.01 as compared to control; ++ = p < 0.01 as compared to Aβ_1–42 alone; n = 4).