Skip to main content

Table 1 Primary antibodies and lectins

From: Distribution of plasma membrane-associated syntaxins 1 through 4 indicates distinct trafficking functions in the synaptic layers of the mouse retina

Antigen Host Dilution Source (catalog #; clone #) Reference
Syntaxin 1 Mouse 1:500–1:1000 Sigma Chemical Company, St. Louis, MO (S0664; clone HPC-1) 57
Syntaxin 2 Rabbit 1:100–1:200 Synaptic Systems, Göttingen, Germany (110-022) 108
Syntaxin 3 Rabbit 1:750–1:1000 Novus Biologicals, Littleton, CO (AB4113) 5,109
Syntaxin 4 Rabbit 1:100–1:200 Chemicon International, Temecula, CA (AB5330) 95,110
Bassoon Mouse 1:500 Stressgen Biotechnologies, Victoria, British Columbia, Canada (VAM-PS003; clone SAP7F407) 111
Calbindin Mouse 1:300 Sigma Chemical Company, St. Louis, MO (C9848; clone CB955) ---
CD15 Mouse (IgM) 1:50 BD Biosciences Pharmingen, San Diego, CA (555400; cloneHI98) 35
Goα Mouse 1:500–1:1,000 Chemicon International, Temecula, CA (MAB3073; clone 2A) 112
Glutamic Acid Decarboxylase, 65 kDa (GAD-65) Mouse 1:500–1:1000 Chemicon International, Temecula, CA (MAB351; clone GAD-6) 113
Glycine transporter 1 (GlyT1) Goat 1:2000 Chemicon International, Temecula, CA (AB1771) ---
Glutamine synthetase Mouse 1:1000 Chemicon International, Temecula, CA (MAB302; clone GS6) 114
Kinesin II Mouse 1:50 Covance Inc., Princeton, NJ (MMS198P; clone K2.4) 115
Microtubule Associated Protein 1 (MAP-1) Mouse 1:300–1:500 Sigma Chemical Company, St. Louis, MO (M4278; clone HM-1) 116
Peanut Agglutinin (PNA) --- 1:10 Molecular Probes, Eugene OR 117
Postsynaptic density protein-95 Kda (PSD-95) Mouse 1:100 Upstate Biotechnologies, Lake Placid, NY (05–494; clone K28/43) ---
Synaptic vesicle protein 2 (SV2) Mouse 1:20–1:100 Dr. K Buckley, Harvard Medical School, Boston, MA 118
Synapsin I Mouse 1:500–1:1000 Chemicon International, Temecula, CA (MAB355) 119
Tyrosine hydroxylase (TH) Mouse 1:500 Chemicon International, Temecula, CA (MAB318; clone LNC1) ---
Vesicular glutamate transporter 1 (VGLUT1) Guinea pig 1:2500–1:5000 Chemicon International, Temecula, CA (AB5905) 28
Vesicular glutamate transporter 3 (VGLUT3) Guinea pig 1:2500 Chemicon International, Temecula, CA (AB5421) 26