Figure 2
![Figure 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1471-2202-7-40/MediaObjects/12868_2006_Article_245_Fig2_HTML.jpg)
ADX increased TGF-β1 NA expression in the hippocampus. Photomicrographs show the changes in TGF-β1 mRNA levels in hippocampus of: (A) control (sham-operated), (B) 2 days after ADX (ADX-2), and (C) 5 days after ADX (ADX-5) animals. The in situ hybridisation was performed using a digoxigenin-labelled oligonucleotide probe complementary to TGF-β1 mRNA (524 pb – 563 bp; accession number NM_021578.1). The specificity of the hybridisation was evaluated using a 100-fold excess of unlabelled oligonucleotide (D). Scale bar = 500 μm.