The rat ZAS3 gene and gene products. (A) A diagonal blot of the rat ZAS3 cDNA against mouse ZAS3 cDNA. (B) Schema of the rat ZAS3 gene (top panel), the complete protein product (middle panel), and a product generated by alternative splicing of exon 13 (bottom panel). White boxes represent exons; vertical arrows indicate two additional exons corresponded to a cDNA sequence present in GenBank databases (XM_233464); asterisk highlights exon 13 that is involved in alternative splicing; horizontal arrows mark the positions of primers used in RT-PCR; and black boxes represent zinc finger pairs. The 5' end of the rat ZAS3 gene has not been elucidated. Therefore, exon numberings, referring to the mouse gene, are shown on top of exons.