Figure 2

Nissl-stained motor neuron count in the lumbar spinal cord. The effect of the KD on Nissl-stained neuronal cell counts in SOD1-G93A transgenic mice at study endpoint. (A) Photomicrographs of representative Nissl-stained sections through the ventral horns of the lumbar spinal cord from wild-type (WT) (a), standard diet- (b) and KD (c) treated SOD1-G93A mice. Horizontal bar = 25 μm. (B) The counts of the large motor neurons in lumbar spinal cord of WT control and of SOD1-G93A treated with standard diet or KD (data = mean ± se; WT: n = 5, Tg G93A: n = 4, Tg G93A+KD n = 6; ## - p = 0.003, Tg G93A vs. Tg G93A+KD; *** - p < 0.001 vs. WT).