Figure 1From: Dendritic mRNAs encode diversified functionalities in hippocampal pyramidal neuronsA schematic diagram of the experiment. Stratum pyramidale and stratum radiatum were dissected from hippocampal slices of adult rats. Total RNA extracted from each fraction was reverse primed with a T7 promoter-conjugated oligo-d(T) primer and labeled with either Cyanine 3 or Cyanine 5 through in vitro transcription. Equal amount of labeled probes were mixed and hybridized to a set of five replicates of the Agilent 22 K rat oligonucleotide microarray. Enlarged views of the microarrays are presented showing reproducible hybridization.Back to article page