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Figure 2 | BMC Neuroscience

Figure 2

From: The neuroblast and angioblast chemotaxic factor SDF-1 (CXCL12) expression is briefly up regulated by reactive astrocytes in brain following neonatal hypoxic-ischemic injury

Figure 2

SDF-1 Antibody Specificity. The polyclonal SDF-1 antibody was tested for non-SDF-1 antigenic interactions and non-specific tissue binding. Panel A demonstrates the SDF-1 antibody's recognition of SDF-1 antigen on mouse hippocampus. Panel B demonstrates that preabsorption of the SDF-1 antibody with recombinant murine SDF-1α completely blocks specific binding of the antibody to the tissue with only very low (diffuse background) non-specific binding. Panel C shows that leaving the primary (SDF-1) antibody incubation step out of the staining protocol does not result in non-specific binding of the secondary antibody or the detection reagents. Scale bar equals 200 μm.

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