Neuronal expression of B3 in adult murine and human cerebellum. (a) Non-radioactive in situ hybridization of PlxnB3 mRNA in adult murine cerebellum using a probe corresponding to nucleotides 4,647 – 5,936 of PlxnB3 cDNA. Purkinje and granular cells show most intense staining (200 × magnification). (b) Detection of plexin B3 protein in adult human cerebellum by immunohistochemistry using antibody pAbB3-B. Positive signal is represented by brown staining and most intense in cerebellar Purkinje cells and neurons of the granular layer (400 × magnification).