Expression of PlxnB3 in cultivated primary murine cerebellar neurons. Expression of PlxnB3 in cultivated primary cerebellar neurons isolated from six days old c57BL/6J mice (a-f; 400 × magnification) but not in astrocytes (g-i) or oligodendrocytes (j-l) (630 × magnification). The cells were cultivated from a cerebellar homogenate of a six days old mouse and analyzed by combined in situ hybridization (ISH) using a PlxnB3-specific probe (a, d, g, j) and immunocytochemistry (IC) using cell type-specific antibodies (b, e, h, k). Overlays of ISH bright field signals and IC signals (blue) are shown in c, f, i, and l. The cells were grown under the respective selective conditions for neurons, astrocytes or oligodendrocytes. Following ISH slides were used for IC using primary antibodies against Neuromodulin (b) or NeuN (e) for neurons, GFAP for astrocytes (h) or CNPase for Oligodendrocytes (k). The Neuromodulin- and NeuN-positive cells (neurons) shown in b and e are positive for PlxnB3 mRNA (a, d).