Brevican and EAMESE Western blotting in regions of mouse brain: Panel A shows an immunoblot containing extracts from various regions of mouse brain and probed with anti-brevican antibody. Immunoreactive bands are present at >145 kD (high molecular weight smear), 145 kD core protein, and the generalized 55 kD fragment. Panel B contains a membrane with the same samples probed with anti-EAMESE. This antibody recognized the specific ADAMTS-derived 55 kD fragment of brevican. CB = cerebellum, BS = brain stem, DE = diencephalon, HC = hippocampus, FC = frontal cortex, TL = temporal lobe, and OT = olfactory tubercle.