Figure 7
Effect of various concentrations of MK-801 on glutamate-induced [Ca2+] i influx. Neuronal cells were maintained for 7 days in conditions similar to those described under Figure 1, prior to loading with 5 μM Fluo-3 AM. After a 15 min treatment with various concentrations of MK-801 cells were treated with 200 μM glutamate. Changes in fluorescence were monitored using the Fluorskan Ascent FL and results were converted to changes in [Ca2+]i (nM) as described in the materials and methods. [Ca2+]i measurements were recorded every 15 secs. Baseline levels of [Ca2+]i were monitored for 60 secs prior to injection with 200 μM glutamate (indicated by glu). Following injection changes in [Ca2+]i were monitored for an additional 120 secs. MK-801 (0 μM) represents 200 μM glutamate alone treatment. Each point represents a mean of 8 replicates. Error bars represent SEM.