Nestin+ and GFAP+ Projections leaving the SVZ. In control brains at P12 and P14 GFAP+ nestin+ projections were seen leaving the SVZ extending towards the cortex (cx; arrows, a). After NMDA-induced excitotoxicity at 3 and 5 days post lesion, the presence of mature astrocytes with a star-like morphology were noticed next to the prolongations (b; asterisks, d). There was also a decrease observed in the number of prolongations (c, d) and orientation seemed disturbed such that many prolongations did not extend towards the cortex or were shorter (arrows, c) and did not reach the outer layers of the cortex (b-d). At 3 and 5 days post lesion note that the GFAP+ and nestin+ prolongations were also seen to extend into the corpus callosum (cc; e) and towards the striatum (see figure 5, b-c) in control brains at P12 and P14. BrdU (red); nestin, GFAP (green).