Figure 6

Annexin A7 immunostaining in human isocortex. (A) Pyramidal neurons (bold arrow) and apical dendrites (small arrows) were clearly labeled with the antibody against Annexin A7 in the human parietal cortex. (B) A dendritic staining was also seen in the molecular layer (small arrows). In addition the subpial astrocytes were weakly labeled (double headed arrows). The staining was cytoplasmic, only few astrocytes showed a nuclear staining as well (inset). (C) Enlargement of (A): Annexin A7 was seen at the cell membrane of the perikaryon (bold arrow) and the apical dendrite (small arrows). (D) A blank control lacking the primary antibody did not show a specific labeling, but autofluorescent lipofuscin was detectable in the neurons (arrowheads). Bar, (A) 70 μm, (B) 50 μm, (B-inset, C) 17 μm, (D) 20 μm.