Figure 1

Characterization of the CHO-K1 cells stably expressing MYC-tagged human DOR. A. The cell surface receptors were visualized with an anti-MYC antibody and a fluorescent secondary antibody. B. The cells were treated with 100 nM DPDPE for 10 min at +37°C after which the remaining cell surface receptors were visualized with an anti-MYC antibody and a fluorescent secondary antibody. C. The cells were stimulated with 100 nM DeltI for 5 min at 37°C, permeabilized with saponin and the receptors were visualized with an anti-MYC antibody and a fluorescent secondary antibody. All the images are 0.5 μm confocal sections at the mid-nuclear level. Representative images are shown. Scale bar 20 μm.