|
Proliferation
|
|
BrdU
|
Mouse IgG
|
1:500
|
Novo Castra (Newcastle, UK)
|
|
BrdU
|
Rat IgG
|
1:500
|
Accurate Chemicals (New York, USA)
|
|
Magnocellular neurons
|
|
OT
|
Rabbit IgG
|
1:2000
|
Produced by G. Alonso
|
|
VP
|
Rabbit IgG
|
1:2000
|
Produced by G. Alonso
|
|
Astrocytes
|
|
GFAP
|
Rabbit IgG
|
1:5000
|
Dakko (Glostrup, Denmark)
|
|
Microglia and endothelial cells
|
|
Lectin from bandeirae
| |
1:2000
|
Sigma
|
|
Simplifolia (IsoB4)
| | | |
|
Oligodendrocyte progenitors
|
|
NG2
|
Rabbit IgG
|
1:500
|
Chemicon (Temecula, USA)
|
|
Vessels
|
|
Rat IgG-Alexa 488 conjugated
|
Goat IgG
|
1:2000
|
Molecular Probes (Eugene, USA)
|
|
EBA
|
Mouse IGM
|
1:5000
|
Sternberger Monoclonals (Lutherville, USA)
|
|
Nestin
|
Mouse IgG
|
1:100
|
Hybridoma Bank (Iowa city, USA)
|
|
Vimentin
|
Mouse IgG
|
1:5000
|
Sigma
|
