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Table 1 Antibodies used

From: Hyperosmotic stimulus induces reversible angiogenesis within the hypothalamic magnocellular nuclei of the adult rat: a potential role for neuronal vascular endothelial growth factor

Marker Species Working dilution Vendor or productor
Proliferation
BrdU Mouse IgG 1:500 Novo Castra (Newcastle, UK)
BrdU Rat IgG 1:500 Accurate Chemicals (New York, USA)
Magnocellular neurons
OT Rabbit IgG 1:2000 Produced by G. Alonso
VP Rabbit IgG 1:2000 Produced by G. Alonso
Astrocytes
GFAP Rabbit IgG 1:5000 Dakko (Glostrup, Denmark)
Microglia and endothelial cells
Lectin from bandeirae   1:2000 Sigma
Simplifolia (IsoB4)    
Oligodendrocyte progenitors
NG2 Rabbit IgG 1:500 Chemicon (Temecula, USA)
Vessels
Rat IgG-Alexa 488 conjugated Goat IgG 1:2000 Molecular Probes (Eugene, USA)
EBA Mouse IGM 1:5000 Sternberger Monoclonals (Lutherville, USA)
Nestin Mouse IgG 1:100 Hybridoma Bank (Iowa city, USA)
Vimentin Mouse IgG 1:5000 Sigma
  1. BrdU: bromodeoxy uridine; EBA: endothelial brain antigen; GFAP: glial fibrillary acidic protein; IgG-IgM: immunoglobulins type G and M; OT: oxytocin; VP: vasopressin.
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