Three dimensional reconstructed confocal microscopy demonstrating colabeling BrdU positive and NeuN positive cell in granule cell layer of HI injured hippocampus (P42). Orthogonal view. The neurons are labeled with NeuN (green), and the BrdU positive cells are shown in red. The square is a the Z slice, the superior rectangle is the X slice, and the right rectangle of the Y slice. A and B show two different cells, both co-labeled with BrdU and NeuN.