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Figure 16 | BMC Neuroscience

Figure 16

From: Glutamate-induced apoptosis in primary cortical neurons is inhibited by equine estrogens via down-regulation of caspase-3 and prevention of mitochondrial cytochrome c release

Figure 16

Effects of 17β-E 2 and Δ8, 17β-E 2 on glutamate-induced Cyto c release into cytosol in primary cortical cells. Primary cortical cells isolated from 19 day old fetal rat brains were cultured for 7 days and treated with 1 mM glutamate alone or in the presence of 17β-E2 and Δ8, 17β-E2. After 6 h, cells were collected and cytosol fraction was prepared as described under "Methods". Cytosolic protein levels of Cyto c were determined by Western blot analysis as described under Figure 6. Actin bands were used to monitor the same blot to verify consistency of protein loading. The content of Cyto c was measured by densitometric scanning of the film. The results are the mean (± SEM) of at least three experiments. a = P < 0.05 compared with control; b = P < 0.05 compared with glutamate treated cells.

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