receptor, nestin and β-tubulin III immunoreactivity in C17.2 NSCs. Cells were maintained in 1% FBS for the number of days indicated, except for those shown in panel E, which were cultured in 10% FBS + 5% HS. (A,B) MT1 in cells maintained for 2 days. (C) Peptide (immunogen) blockade of MT1 immunoreactivity after 2 days. (D,E) Nestin immunoreactivity after 2 and 3 days, respectively. (F) β-tubulin III immunoreactivity after 3 days. Scale bars: (A,D,F) 50 μm. (B,E) 20 μm.