Gene | Sequence (5'-3') | Position | Annealing temp (°C) |
---|---|---|---|
Smad2 | TCAGTGCGATGCTCAAGCATGTCC | 1515-1538 | 66 |
AGACCAAGCAGCAGCACACACCTC | 1926-1903 | ||
Smad3 | ACGAGCTGAACCAGCGAGTTGG | 1006-1027 | 45 (5 cycles) |
TGGTGCACATGCGAGTCAACTGG | 1407-1385 | 57 (35 cycles) | |
Smad4 | ATGCCCATTGCGGACCCACAG | 1755-1775 | 66 |
GGAAGACTGGACTGCCACTTGCG | 2253-2231 |