Overlap in hypothalamus of serial pathways from prefrontal area 12 and the intermediolateral spinal column. (A) The bidirectional tracer fluororuby (fr) was injected in prefrontal area 12 (red area). (B) The injection of the retrograde tracer fast blue (fb) was in the spinal cord, covering the intermediolateral cell column, the lowest central autonomic center (blue area). Fast blue labeled neurons projecting to the spinal cord (blue dots) and labeled axons (brown lines) originating in area 12 were intermingled in the following hypothalamic areas: (C) dorsal hypothalamic area (DA) and tuberomammillary nucleus (TM); (D) perifornical nucleus (Pef); (E) fields of Forel (FF). (F) In the same case, labeled neurons in the basolateral (BL) and lateral (L) nuclei of the amygdala projected to area 12 (red dots).