Figure 6From: ALS-associated mutant SOD1G93A causes mitochondrial vacuolation by expansion of the intermembrane space and by involvement of SOD1 aggregation and peroxisomesPeroxisomes participate in mitochondrial vacuolation. Spinal cord sections from SOD1G93A mice were stained with anti-SOD1 (red) and anti-catalase (green) antibodies (A-H), or anti-SOD1 (red) and anti-PMP70 (green) antibodies (I-L), and shown as 3-D reconstructed confocal images. C, G and K are superimpositions of the red and green signals. D and H are the same as C and G, respectively, but rotated to the right for 30°. L is the same as K but rotated for 90°. Arrows in B-D and F-H point to small, catalase-positive granules that line against the inner wall of vacuoles. Arrows in J-L point to small, PMP70-positive granules that line against the inner wall of vacuoles. Notice one PMP-70 granule in J and K represents two granules when viewed at the 90° in L. Unfilled arrows point to granules that lie against the outer wall of the vacuoles.Back to article page