Figure 1
From: Insulin signaling inhibits the 5-HT2Creceptor in choroid plexus via MAP kinase
Effect of insulin and PD 098059 on the response to serotonin in rat choroid plexus cells. (A) Change in intracellular calcium in response to 5-HT in a representative choroid plexus cell. Horizontal bar above the trace indicates bath application of 2 μM 5-HT. (B) Change in intracellular calcium in response to 5-HT after 10 minute treatment with insulin in a representative choroid plexus cell. Horizontal bars above the trace indicate bath application of 10 μM insulin or 2 μM 5-HT. (C) Change in peak intracellular calcium concentration in response to 5-HT with and without insulin treatment and/or PD 098059 pretreatment. Basal intracellular calcium levels averaged 20-50 nM for all groups. The data are presented as mean ± S.E.M. * indicates a statistical difference (P < 0.05) of the insulin treatment group when compared to control.