Model of α
-GFP(92–94). The GFP insertions into αs can be interpreted in the context of the structures of GFP (PDB file: 1EMA) and αs-GTPγ S (PDB file: 1AZT). In this image, the structure of GFP  is green, while the helical domain of the αsubunit  is pink, and the GTPase domain is blue. GTPγ S is yellow. The GFP insertion αs-GFP(92–94) that produced a functional G protein subunit is illustrated by the short linkers (encoded by the Tn5 MEs) between GFP and αs (dark blue). The other sites of <EGFP-V> insertion are shown as green spheres. The numbers on the spheres indicate the second of the three duplicated residues that flank the transposon insertions (the numbers are based on the long form of αs).