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Figure 1 | BMC Neuroscience

Figure 1

From: The predominantly HEAT-like motif structure of huntingtin and its association and coincident nuclear entry with dorsal, an NF-kB/Rel/dorsal family transcription factor

Figure 1

Concomitant nuclear accumulation of the carboxyl-terminal fragment of Drosophila huntingtin and dorsal. a,b, When pPac-FLAG-Dorsal encoding FLAG-tagged dorsal was transfected into S2 cells, approximately half of the transfected cells had predominantly cytoplasmic distribution of FLAG-Dorsal ( a ) and the remaining half showed predominantly nuclear distribution ( b ). FLAG-Dorsal was detected by anti-FLAG M5 antibody and Alexa Fluor 488 goat anti-mouse IgG. DNA staining with TOTO-3 determined the nuclei. c, When pIZ-Dhd2194C-myc encoding the myc-tagged carboxyl-terminal fragment of Drosophila huntingtin, Dhd2194C-myc, was introduced, the product was predominantly distributed to the cytoplasm. As a control, nuclei were determined by co-transfection of pIZ-NLS-GFP-lacZ encoding a SV40 nuclear localization signal-green fluorescence protein-lacZ chimeric protein [49] detected by anti-lacZ antibody and Alexa Fluor 488 goat anti-mouse IgG. Dhd2194C-myc was detected by anti-myc antiserum A-14 and Alexa Fluor 568 goat anti-rabbit IgG. d, Co-expression of FLAG-Dorsal and Dhd2194C-myc causes prominent nuclear accumulation of both proteins. The same observations were made using pIZ-HA-Dorsal and pIZ-FLAG-Dorsal (data not shown).

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BMC Neuroscience

ISSN: 1471-2202

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